Figure 9.

Endogenous Myo2p does not coimmunoprecipitate with soluble HA-tagged overexpressed tail. MYO2DN cells were grown in galactose for 1 h. The postnuclear supernatant was spun at 100,000 × g for 1 h, resulting in S100 and P100. The monoclonal anti-HA antibody, 12CA5, was used to immunoprecipitate the HA-tagged tail from both S100 and P100. The immunoprecipitated material (Pellet) and unbound material (Sup) were loaded onto SDS-PAGE gels using volumetric stoichiometry. Immunoblots were performed using anti-Myo2p motor-domain antibody (top panel) or anti-HA polyclonal antibody (lower panel).