Abstract
Ultraviolet (UV) microscopy was used to study the capacity of yeast (ATCC 10231 and 10261) and filamentous (ATCC 10259) strains of Candida albicans to accumulate UV-absorbing materials from a medium supplemented with purines, pyrimidines, amino acids, or related compounds as the main nitrogen source. All strains accumulated UV-absorbing compounds when adenine, adenosine, isoguanine, xanthine, or uric acid was supplied as a nitrogen source, but they did not accumulate UV-absorbing compounds when pyrimidines were supplied. The filamentous strain accumulated UV-absorbing material from medium supplemented with hypoxanthine, but the yeast strains did not. In contrast, the yeast strains accumulated more UV-absorbing material than did the filamentous strain when guanine was the nitrogen source. Yeast strain 10231 not only accumulated UV-absorbing material from tyrosine-supplemented medium, but it became filamentous in form as well. Yeast strain 10261 and filamentous strain 10259 did not accumulate detectable amounts of UV-absorbing material, nor was their morphology noticeably affected by the supplement. The two yeast strains accumulated more lipid than the filamentous strain when they were incubated in a nitrogen-deficient medium.
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