Figure 2.

Cell-free assays of S. pombe GT expressed in S. cerevisiae. Microsomes from S. cerevisiae alg6 (PRY103) mutant cells transformed with p416GPD-gpt1⁺ (A) or p426GPD-gpt1⁺ (B) were incubated with UDP–[¹⁴C]glucose in the presence of 8 M urea-denatured thyroglobulin. Resulting Endo H-sensitive oligosaccharides were run on paper chromatography with solvent A. In panel C, thyroglobulin or detergent was omitted where indicated. Standards: 1, Glc₁Man₉GlcNAc; 2, Glc₁Man₈GlcNAc; and 3, Glc₁Man₇GlcNAc.