Figure 5.

GT expression in GII minus cells. Alg5 (OCY2) or gls2-alg5 (OCY3) cells transformed with p425GPD-gpt1⁺ or p425 GPD were incubated for 15 min with [¹⁴C]glucose in the presence or absence of 5 mM DNJ. Endo H-liberated compounds were run on paper chromatography with solvent A. Material migrating between mid-Man₉GlcNAc and Man₈GlcNAc was submitted to strong acid hydrolysis and run on paper chromatography with solvent C. (A) alg5 cells with p425GPD-gpt 1⁺ incubated with DNJ and (B) gls2-alg5 with p425GPD-gpt⁺ cells incubated without DNJ. (C) alg5-gls2 cells with p425GPD incubated with DNJ. Standards: 1, mannose; and 2, glucose.