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Northern hybridization of Paramecium RNA. Total RNA (10 or 15 μg/lane) was prepared from P. multimicronucleatum (A) and P. caudatum (B) and electrophoresed. Probes for hybridization were the 0.87-kbp p36-C fragment of the P. multimicronucleatum C5 antigen cDNA (lane 1), the 0.5-kbp P. caudatum genomic DNA encoding glyceraldehyde-3-phosphate dehydrogenase (lane 2), and the 0.4-kbp P. caudatum hemoglobin cDNA (lane 3).
