Figure 4.

Protein translocation across the ER membrane does not require LAG1 and DGT1. Cells of W303-1B (wild type), WBY616 (lag1Δ dgt1Δ), or DNY116 (sec61-101) were pulse labeled with [³⁵S]methionine and [³⁵S]cysteine for 5 min. Cell lysates were prepared, and immunoprecipitations were performed using polyclonal antibodies against the following proteins: (A) Kar2p, (B) dipeptidyl aminopeptidase B (DPAP B), (C) CPY, and (D) the GPI-anchored cell surface protein Gas1p. Eluates were subjected to SDS-PAGE followed by fluorography. Cytosolic precursor (p) and ER-glycosylated (g) forms of each protein are indicated.