Figure 6.

Growth of cells containing mutant Sla1 proteins. (A) Cells in which the genomic copy of SLA1 was deleted (KAY14) were transformed with the sla1 mutant constructs and plated onto selective medium at 25° and 37°C. (B) Cells carrying ABP1 on a URA3-marked plasmid (KAY78) were transformed with the sla1 mutant constructs and tested for growth on selective medium in the presence or absence of 5-FOA. (C) Cells (KAY14) were cotransformed with sla1 mutant constructs Sla1-ΔC-term.rpts and Sla1ΔGap1+SH3#3 and tested for growth at 37°C. Expression of both forms of Sla1p was verified by Western blotting.