Figure 8.

Rhodamine–actin incorporation into permeabilized cells expressing mutant forms of Sla1p. Cells expressing (A) wild-type Sla1p, (B) no Sla1p, (C) Sla1-ΔSH3#1&2, (D) Sla1-ΔG1+SH3#3, (E) Sla1-ΔC-term. repeats were grown, permeabilized, and tested for actin assembly as described by Li et al., (1995) (see MATERIALS AND METHODS). Sites of incorporation were then viewed by fluorescence microscopy, and the intensity of fluorescence in the buds was measured. The units of fluorescence intensity are arbitrary. Bar, 5 μm.