Figure 2.

GFP–Yck2p fluorescence is plasma membrane-associated and is dynamic during the cell cycle. GFP–Yck2p fluorescence (A–H) and corresponding DAPI staining (A′–H′) were observed for cells of strain LRB859 (diploid GFP:YCK2 rho⁻ strain) grown at 30°C in synthetic medium to a density of 2 × 10⁷ cells/ml. Cells were observed in 10 mM HEPES, pH 7, containing 5% glucose. The cell shown in each pair of panels represents a different cell cycle position, but each pair depicts a different cell. Bar, 2 μm.