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Mutation of the C-terminal Cys codons of GFP–Yck2p abolishes plasma membrane localization. Cells of strain LRB758 (YCK+) carrying pJB2 (pGal1: GFP:YCK2Cys545,546Ser) were grown in selective medium containing raffinose to log phase. Galactose was added to the culture to induce synthesis of the mutant Yck2 protein, and cells were observed in synthetic medium directly on slides after 90-min incubation. Bar, 2 μm.
