Figure 7.

Yck-deficient cells show a hyperpolarized mating response. Cells of strains LRB758 and LRB759 (A; MATa YCK⁺ and MATα YCK⁺, respectively) and cells of strains LRB756 and LRB757 (B; MATa yck^ts and MATα yck^ts, respectively) were mixed and incubated for 2 h at 24°C on rich medium. Mating mixtures were suspended in rich medium and placed on slides for photography using DIC optics. Panels show representative cell morphologies that were quantitated to yield percentages of different morphological types among the unbudded cells in the mixture (n = 130, YCK⁺; n = 79, yck^ts). Bar, 2 μm (magnification in all panels is identical).