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Effect of EGCG on the PDGF-BB–induced DNA synthesis. VSMCs in 24-well plates were precultured in serum-free medium in the presence and absence of different concentrations of EGCG for 24 h. Then the medium was replaced with serum-free medium without EGCG, and VSMCs were stimulated with 50 ng/ml PDGF-BB. After 20 h, 3 μCi/ml of [3H]thymidine were added to the serum-free medium. Four hours later, experiments were terminated. *p < 0.05 for EGCG+PDGF-BB vs. PDGF-BB effect.
