Figure 6.

Overexpression of STE12 does not restore invasiveness to yeast with a deletion of FLO11. Invasive growth of the following strains were compared: wild-type strain L5487 (first row of colonies); L5487flo11–4 (L5487 with a deletion of FLO11) transformed with the 2μ vector control pRS426 (middle row of colonies); and L5487flo11–4 transformed with a multiple copy (2μ) plasmid carrying STE12 (pSY2). Cells were patched onto YEPD medium and grown for 3 d at 30°C followed by 2 d of growth at room temperature. (A) The total growth on the surface of the agar, shown before the plate was rinsed with water. (B) The same plate after rinsing cells off the surface of the agar with a gentle stream of deionized water. Invasive growth is demonstrated by cells that remain in the agar.