Table 1.
Strains and plasmid list
| Strain or Plasmid | Genotype or Description | Reference Source Derivation |
|---|---|---|
| S. cerevisiae strain | ||
| L5486 | MATa, ura3-52, leu2::hisG | Roberts and Fink (1994) |
| L5487 | MATα, ura3-52, leu2::hisG | Roberts and Fink (1994) |
| 10560-4A | MATa, ura3-52, leu2::hisG, | Laboratory of G. Fink |
| trp1::hisG, his3::hisG | ||
| 10560-6B | MATα, ura3-52, leu2::hisG, | Laboratory of G. Fink |
| trp1::hisG, his3::hisG | ||
| YSL1012 | MATa/MATα, ura3-52/ura3-52, | This work. L5487 × YIY318 |
| LUE2/leu2::hisG [URA3] | (Yamashita et al., 1985) | |
| + plasmid pRS426 | ||
| (Christianson et al., 1992) | ||
| YSL1015 | MATa/MATα, ura3/ura3-52, | This work. 10560-4A × |
| leu2::hisG/leu2::hisG | 10560-6B + plasmid pRS423 | |
| trp1::hisG,/trp1::hisG | + plasmid pRS424 | |
| his3::hisG/his3::hisG [TRP1][HIS3] | Christianson et al. (1992) | |
| L5487flo11-4 | MATα, ura3-52, leu2::hisG, | This work |
| flo11::URA3 | Derived from L5487 | |
| YSL12 | MATα, ura3-52, leu2::hisG, | This work |
| ste12::LEU2 | Derived from L5487 | |
| Plasmids | ||
| pRS315-a1 | CEN-LEU2 plasmid carrying MATa1 | This work |
| pRS415-α2 | CEN-LEU2 plasmid carrying MATα2 | Sharon Y. Roth |
| pYSL12 | 2μ-LEU2 plasmid carrying FLO11 | This work |
| pYSL13 | 2μ-URA3 plasmid carrying FLO11 | This work |
| pSL35 | pGEM3Z(f+) containing a 746-bp | This work |
| HindIII–PvuII FL011 fragment | ||
| pGEM-ACT300 | pGEM3Z containing a 300-bp BalII | K. Wang; Ng and Abelson, 1980 |
| fragment from pYACT1 | ||
| pSUL16 | ste12::LEU2-containing plasmid | Fields and Herskowitz, 1987 |
| pSY2 | 2μ-LEU2 plasmid carrying STE12 | Dolan and fields, 1990 |