Figure 1.

Positively charged Nanogold can be used as an endocytic marker. Wild-type and end3 mutant strains were grown overnight, converted to spheroplasts, and incubated under various conditions on ice for 15 min followed by a 30-min incubation at RT. The samples were fixed and embedded and thin sections were cut. (A) Sections from wild-type cells incubated with positively charged Nanogold were enhanced with HQ Silver. (B) Sections from end3 mutant cells incubated with positively charged Nanogold and enhanced. (C) Sections from wild-type cells incubated in the absence of positively charged Nanogold, but enhanced. (D) Sections from wild-type cells incubated with positively charged Nanogold in the presence of NaN₃ and NaF and enhanced. The cells were visualized in the electron microscope as described in MATERIALS AND METHODS. Bar, 200 nm.