Figure 10.

Small endocytic vesicles accumulate in sec18 spheroplasts. Mutant (sec18) cells were grown overnight at 24°C, converted to spheroplasts, and incubated with positively charged Nanogold on ice for 15 min and subsequently warmed up to RT or nonpermissive temperature (32°C) for 0, 5, 10, or 40 min. Labeled vesicles, vesicular/tubular structures (early), and oval or spherical structures (late) were counted from 20 spheroplast sections at each time point and temperature.