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Quantitation of internalization by end3 spheroplasts. Positively charged Nanogold was incubated with wild-type and end3 spheroplasts as described in Figure 1, A and B. Gold particles were counted for five sections each on the plasma membrane and over the cell interior. The percentage of internal gold particles was calculated and plotted. The total number of cell surface particles counted was 3680 for wild-type spheroplasts and 4692 for end3 spheroplasts.
