Figure 9.

Small endocytic vesicles are seen in sec18 spheroplasts. Wild-type (A) or sec18 (B and C) cells were grown overnight, converted to spheroplasts, preincubated at 32°C for 10 min, and then incubated with positively charged Nanogold at 32°C for 30 min. The spheroplasts were prepared for electron microscopy and visualized as for Figure 1A. Bar, 200 nm.