Figure 2.

β-Catenin migrates into the nucleus of living cells in a temperature-dependent and WGA-sensitive manner. Purified recombinant mouse FLAG-β-catenin (1 mg/ml) was injected into the cytoplasm of MDBK cells with (e and f) or without (a–d) 2 mg/ml WGA. After incubation for 30 min at 37°C (a, b, e, and f) or on ice (c and d), cells were fixed with 3.7% formaldehyde in PBS. Injected FLAG-β-catenin was visualized by indirect immunofluorescence with anti-FLAG mouse mAb and CY3-labeled anti-mouse goat IgG. The localization of β-catenin was examined by Axiophoto microscopy (Zeiss).