Figure 5.

Intracellular distribution and phosphorylation of enlarged Hog1 kinases. (A) Fluorescence images of hog1 cells transformed with a plasmid expressing Hog1 fused to three tandemly repeated GFP polypeptides. Cells were treated and processed as in Figure 8. GFP images were taken under normal growth conditions 5 min after osmotic stress and 5 min after return to iso-osmotic medium. (B) Western analysis from yeast cells containing a HOG1 fusion with one, two, and three copies of GFP. The top panel was been probed with anti-active p38 antibody; the bottom panel was probed with antibodies against GFP. The apparent sizes for marker proteins are indicated.