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. 1999 Apr;10(4):1163–1178. doi: 10.1091/mbc.10.4.1163

Figure 5.

Figure 5

C5aR-GFP dynamics after stimulation of PLB-985 cells with a point source of ChaCha delivered from a micropipette. C5aR-GFP-expressing cells were differentiated with 1.3% DMSO and plated on glass coverslips. Cells were then stimulated with ChaCha (100 μM) delivered from a micropipette, and images were recorded every 2 s, under pseudoconfocal conditions, as described in MATERIALS AND METHODS. Responses are shown after (A) 0, (B) 44, (C) 110, (D) 154, (E) 176, and (F) 220 s of micropipette stimulation (white dot). The closed arrowhead in panel A points to the retraction fibers of cell b. Open arrowheads (panel B) point to ruffles at the leading edges of locomoting cells. Arrows in panels C and E point at the back of polarized cells. Red arrowheads (panel F) point to internalized C5aR–GFP. This session is representative of three similar observations. Bar, 10 μm. A video of the experiment described in this figure is available on the internet version of this paper, at http://www.molbiolcell.org.