Figure 6.

Localization of C5aR–GFP relative to the plasma membrane of moving PLB-985 cells. C5aR-GFP–expressing cells were differentiated with DMSO 1.3%, labeled with DiD, and plated on glass coverslips. Cells were then stimulated with ChaCha (100 μM) delivered from a micropipette. Under pseudoconfocal conditions, the GFP and DiD signals were alternatively recorded in the FITC and Texas Red channels, respectively, as described in MATERIALS AND METHODS. Single cells, from three different experiments, are shown crawling toward the source of ChaCha (white dot). Arrows point to the ruffling fronts of the cells. Bar, 10 μm.