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. 1998 Jan;9(1):209–222. doi: 10.1091/mbc.9.1.209

Figure 3.

Figure 3

(A) Identification of the Der3 protein. Crude extracts of exponentially growing cells on CM medium of strains W303–1C [wild type (WT)], W3031-C harboring the high-copy plasmid YEpDER3, YAF29 (der3–1), W303–1CΔ3 (Δder3), and W303–1CD (Δder1) were prepared, separated by SDS-PAGE, and immunoblotted. Levels of Der3p were detected with polyclonal Der3p antisera. (B) Der3p is an integral membrane protein. Crude extracts enriched in membranes were prepared from exponentially growing cells on CM medium of strains W303–1C (WT) and W303–1CΔ3 (Δder3). Aliquots of the soluble (S) and membrane (P) fractions were loaded onto a 8% SDS-polyacrylamide gel and separated by electrophoresis followed by Western blotting. Der3p antigenic material was visualized with anti-Der3p polyclonal antibodies.