Figure 6.

Triton X-114 partitioning of monomeric and T7-REP-associated Myc-Rab1B pools. 293 cells were cotransfected with plasmids encoding Myc-Rab1B(wt) and T7-REP and maintained in medium containing 10 μM lovastatin for 48 h. Cytosol was fractionated on a Superose-12 column (upper panels) and fractions containing Myc-Rab1B that eluted with T7-REP (pool 1) or at the position of the 29-kDa marker (pool 2) were combined as indicated. Both pools were subjected to the Triton X-114 phase partitioning assay described in MATERIALS AND METHODS. The proteins in the resulting aqueous and detergent phases were run on SDS gels and immunoblotted with the anti-Myc antibody (bottom panels).