Figure 1.

Western blots of cytoplasmic dyneins from rat brain and testis. Three identical blots containing cytoplasmic dyneins isolated from rat brain (B) and rat testis (T) were resolved by 6.7% SDS-PAGE, transferred to nitrocellulose, and probed with antibodies to DHC1a (anti-1a) and DHC1b (anti-1b) and with the pan-reactive anticytoplasmic dynein antibody (JR). Blots were developed with horseradish peroxidase–conjugated secondary antibodies, followed by chemiluminescence and exposure to x-ray film. The anti-1b-immunoreactive protein from testis, but not brain, was smaller than DHC1a.