Figure 2.

Northern blots of rat testis RNA. Twenty micrograms of testis poly(A)⁺ RNA were resolved on a 0.8% agarose gel under denaturing conditions. Samples were blotted onto membranes and hybridized overnight with ³²P-labeled 300-bp cDNA probes derived from DHC1a and DHC1b. Identical strips were individually probed with one of the cDNAs, and the strips were exposed side by side to the x-ray film. (A) Autoradiogram after low-stringency wash (100 mM sodium phosphate, 50°C). (B) Autoradiogram of the same blots after high-stringency wash (50 mM sodium phosphate, 65°C). The DHC1b transcript is smaller and appears to be less abundant than the DHC1a mRNA.