Figure 4.

Western blot of dynein intermediate chain IC74 in the sucrose gradient fractions. The odd-numbered fractions (3–29) from a sucrose gradient were resolved on an 8% SDS-polyacrylamide gel and blotted to nitrocellulose. In this gradient, DHC1a was present in fractions 17–23, with its highest concentration in fractions 19–21, and DHC1b was present in fractions 11–18. The blot was probed with anti-IC74, followed by horseradish peroxidase–conjugated secondary antibody and chemiluminescence. The IC74 was associated with DHC1a (fractions 17–23) but did not associate with most of the anti-1b-immunoreactive protein (fractions 11–17).