Figure 4.

Expression of germ cell-specific isoforms of HK1 during spermatogenesis. Proteins (40 μg) from pachytene spermatocytes (PS), round spermatids (RS), condensing spermatids (CS), and whole sperm (“sperm”) were separated using SDS-PAGE. The appropriate purified recombinant proteins were used as positive controls. (A) α-sa immune and α-sa preimmune antisera. (B) α-sb immune and α-sb preimmune antisera. (C) α-gcs immune and α-gcs preimmune purified antisera. The first appearance of HK1-sc in round spermatids matches the previously established expression pattern of HK1-sc mRNA (Visconti et al., 1996).