Figure 5.

Expression of germ cell-specific isoforms of HK1 in somatic tissues. Forty micrograms of protein extracts from various somatic tissues were prepared and separated using SDS-PAGE and then subjected to immunoblotting with the α-gcs immune and α-gcs preimmune antisera. Immunoblots with the α-sa and α-sb antisera are not shown but yielded identical results. Reprobing of these blots with the α-HK1 antiserum capable of recognizing the somatic isoform revealed that the somatic isoform was detectable in a variety of tissues (our unpublished results).