Figure 2.

Reversed-phase HPLC purification of RNase 2.4. Immunopurified RNase 2.4 was chromatographed on a C₄ column (1 mm in diameter) equilibrated in 0.1% TFA. A linear gradient of 0–80% solvent B (70% CH₃CN in 0.085% TFA) over 75 min was used at a flow rate of 50 μl/min. The inset shows a Western blot analysis of the fractions using antibodies against RNase 4 or modification-specific antibodies, α(5–10).