Figure 3.

Western blot analysis of single-site mutants of RNase 2.4. Approximately 0.5 μg of each protein was electrophoresed on a 15% SDS-PAA gel and blotted onto nitrocellulose. The blots were probed with modification-specific antibodies, α(5–10), and, after stripping, with αRNase 4 antibodies.