Figure 5.

Mutational analysis of RNase 2.4 and RNase 2. (A) The degree of C-mannosylation of Trp-7 in the indicated mutants of hybrid RNase 2.4 was determined from the ratio of modified and unmodified fragments −4 to 12 and is depicted by filled bars. (B) The degree of modification of Trp-7 in the indicated mutants of RNase 2 was determined as in A and are plotted as open bars for the experiment performed in 3T3 cells and as filled bars for the experiment performed in HEK293 cells. The data represent the average of at least two independent experiments. The SD was 1–16% of the mean.