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. Author manuscript; available in PMC: 2008 Aug 26.
Published in final edited form as: Biochemistry. 2006 Mar 21;45(11):3769–3774. doi: 10.1021/bi052600m

Figure 2.

Figure 2

EC50 for the ability of sTF-His to enhance the rate of fX activation by fVIIa. Varying concentrations of purified sTF or sTF-His were incubated in wells of 96-well plates that had previously been coated with a mixture of either 10% DOGS-NTA-Ni, 20% PS, 70% PC (closed symbols) or 20% PS, 80% PC (open symbols). Initial rates of fXa generation were measured using 40 nM fX and are expressed as percent of the activity observed with 1 μM sTF-His in wells coated with lipid mixtures containing DOGS-NTA-Ni. (a), Concentration dependence of the ability of sTF-His (circles) or sTF (squares) to enhance the rate of fX activation by fVIIa. (b), Data for sTF-His from panel a are plotted with the x-axis expanded from 0 to 50 nM.