Figure 8.

(A) Far-UV CD spectra of MAGI1 WW2 355–390 (black), MAGI1 WW2 355–401 (red), and MAGI1 WW2 355–390 D370S (green), recorded at 20°C. (B) Thermal denaturation profiles of MAGI1 WW2 355–390 (black), MAGI1 WW2 355–401 (red), and those of the D370S mutant, recorded with 55 μM (green) and 5 μM (blue) protein concentrations. The changes in ellipticity at 232 nm for MAGI1 WW2 355–390 and MAGI1 WW2 355–390 D370S, and that at 217 nm for MAGI1 WW2 355–401, were monitored and analyzed as a function of temperature.