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Analysis of N-terminal fusion tags with sPLA2-IIE, sPLA2-III, and sPLA2-V. (A) sPLA2-IIE samples. (B) sPLA2-III samples. (C) sPLA2-V samples. Media samples (15 μL), 48 h post-transfection (HEK-293T), were separated by SDS-PAGE and transferred to nitrocellulose. Blots were probed with monoclonal anti-6xHis. Results are representative of two to three independent experiments.
