Figure 2.
5-azacytidine treatment increases IFN-γ and IL-3 secretion by primary mouse CD8+ T lymphocytes. CD8+ T cells from normal C57BL/6 mouse LNs were activated in low density in vitro cultures using solid-phase mAb against CD3ε, CD8, and LFA-1 in the presence of IL-2 and varying concentrations of 5-azacytidine for the indicated periods of time. Cumulative secreted cytokine levels were quantitated by ELISA (A, IFN-γ) or reporter cell bioassay (B, IL-3). The results shown are representative of three independent experiments.