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. 1998 Jul 1;188(1):103–117. doi: 10.1084/jem.188.1.103

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Quantitation of IFN-γ and IL-3 mRNA levels in a panel of CD8⁺ clones by competitive PCR after 4–5 d of stimulation. Levels of mRNA were determined by QCPCR and corrected for CD3ε mRNA levels as described in Materials and Methods. A shows relative IFN-γ mRNA levels in relation to clone size. B shows relative IL-3 mRNA levels in relation to clone size in the same set of clones. C shows the relationship between IFN-γ and IL-3 expression levels for each clone in the panel. The results for 53 clones are shown.

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