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. 1998 Jul 1;188(1):49–60. doi: 10.1084/jem.188.1.49

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SDS-PAGE and silver staining of LPS from L. pneumophila SG 1. 2 μg purified LPS were applied to each lane. Lane 1, mutant 811; lane 2, wild-type RC1; lane 3, strain Philadelphia. All strains exhibit a characteristic bimodular distribution of LPS O-chain representing 10–35 and 45–100 carbohydrate units, respectively. On the left side, LPS bands corresponding to fractions I and II of the gel filtration on Sephadex columns are indicated. These fractions were employed for epitope mapping by competition ELISA. As a control, 2 μg purified LPS from P. aeruginosa subgroup Fisher 2 was applied to lane 4.