Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1998 Jul 1;188(1):211–216. doi: 10.1084/jem.188.1.211

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Inhibition of apoptosis by ectopic xiap expression. (A) Lysates of noninfected or infected HUVECs were separated by SDS-PAGE, blotted onto nylon membranes, and stained for myc-tagged XIAP protein. AdV, adenovirus; GFP, green fluorescent protein. (B) HUVECs were infected with IκBα alone (c and d), together with xiap (e and f ), or together with GFP (g and h) recombinant adenovirus. 48 h after infection cells were treated with TNF-α (500 U/ml) for 6 h or left untreated and analyzed by FACS^® after propidium iodide staining. Cells with a DNA content <2 N appear in the sub-G1 region (M1). The percentage of cells found in the M1 region is indicated. The data show one out of three representative experiments.

Inhibition of apoptosis by ectopic xiap expression. (A) Lysates of noninfected or infected HUVECs were separated by SDS-PAGE, blotted onto nylon membranes, and stained for myc-tagged XIAP protein. AdV, adenovirus; GFP, green fluorescent protein. (B) HUVECs were infected with IκBα alone (c and d), together with xiap (e and f ), or together with GFP (g and h) recombinant adenovirus. 48 h after infection cells were treated with TNF-α (500 U/ml) for 6 h or left untreated and analyzed by FACS^® after propidium iodide staining. Cells with a DNA content <2 N appear in the sub-G1 region (M1). The percentage of cells found in the M1 region is indicated. The data show one out of three representative experiments.