Figure 5.

Chemokine expression after chemokine blockage. On day 21, total RNA from lungs of OVA plus Ab control–, OVA plus anti-Eot–, OVA plus Met-RANTES–, or OVA plus anti–MCP-1–treated mice was extracted 3 h after antigen challenge. Chemokine blockage was performed daily before OVA provocation on days 8–21. In a control group, mice were treated with PBS (intranasally) and the irrelevant Ab (intravenously) instead of OVA (intranasally) and the chemokine-neutralizing Ab (intravenously), respectively. RANTES, eotaxin, and MCP-1 mRNA expression were determined by RPA. Both TCA and GAPDH mRNA expression were measured as negative and positive control, respectively. The intensity of the band for each chemokine was normalized to the intensity of the band of GAPDH in the control sample (not shown) and analyzed. Chemokine expression of two representative mice out of four is shown.