Figure 5.

Chemotactic activity of ELC on resting and acutely activated mouse lymphocytes. Results are expressed as the percentage of input cells of each subtype migrating to the lower chamber of a transwell filter. Panels show migration of spleen lymphocyte subsets: (A) CD4 T cells, CD8 T cells, and B cells; (B) L-selectin^hi and L-selectin^lo CD4 T cells; (C) B cells preincubated with anti-IgM (17 μg/ml) or media alone (control) for 4 h; (D) duplicate experiment to C with cells preincubated with media alone (−) or anti-IgM (aμ) for 4 h; (E) purified B cells preincubated with media alone (−), anti-IgM at 17 μg/ml (aμ), or LPS at 20 μg/ml (L) for 6 h; (F) total spleen cells in the absence of a gradient (no gradient, equal concentration of ELC in upper and lower chamber) or preincubated with pertussis toxin at 200 ng/ml (PTX) for 2 h. In A–E, SDF1α was included at 300 ng/ml as a positive control. In D, ELC was at 200 ng/ml, and in E and F at 300 ng/ml. The results in A–C are representative of three independent experiments, and results in D–F of two experiments.