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. 1998 Jul 1;188(1):181–191. doi: 10.1084/jem.188.1.181

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Chemotactic activity of ELC on immature T and B cells and lack of activity on granulocytes and macrophages. Results are expressed as the percentage of input cells of each subtype migrating to the lower chamber of a transwell filter. Panels show response of (A) mature CD4 and CD8 single positive (SP) thymocytes; (B) immature CD4/CD8 double negative (DN) thymocytes, total CD4/CD8 double positive (DP) thymocytes, and CD3^hi double positive thymocytes; (C) bone marrow B220+IgM−IgD− cells (pre-B), B220+IgM+IgD− cells (immature B), and B220+IgM+IgD+ cells (mature B); (D) bone marrow granulocytes; and (E) spleen macrophages. Insets in A and C show flow cytometric profiles of input cells and gates used to measure the frequency of each cell type. The profile in C has already been gated for B220+ cells. The inset in B shows the gate used to identify high CD3 expression on double positive thymocytes and, as an example, the fraction of CD3^hi double positive cells that migrated in the absence of ELC or in response to 1 μg/ml ELC. In D and E, SDF1α (300 ng/ml) is included as a positive control. Each experiment was performed a minimum of two times.