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Surface expression of biotinylated HeV F proteins. Wt HeV F and the CBF1 mutants were expressed in BSR cells. Cells were labeled overnight with Tran[35S] and chased for 2h. Samples were biotinylated and immunoprecipitated as total and surface populations as described in Experimental Procedures. Samples were separated on a 15% SDS-polyacrylamide gel under reducing conditions and analyzed by use of the Storm or Typhoon system.
