Figure 5.

Oligomerization of the CBF₁ mutants. Wt SV5 F (A) or HeV F (B) and the CBF₁ mutants were expressed in HeLa T4 cells by use of the vTF7-3 system. Three h post-transfection, cells were starved for 30 min, pulsed with Tran[³⁵S] for 30 min and chased for 1 h. Chemical cross-linking was performed on cell suspensions in the presence of 0.2% NP-40 and 1mM DTSSP. Samples were immunoprecipitated, analyzed on a 3.5% polyacrylamide gel in the absence of a reducing agent and visualized by use of the Storm or Typhoon system.