Figure 7.

Expression of the SV5 CBF₁ mutants at a reduced temperature. Wt SV5 F and the CBF₁ mutants were expressed in BSR cells. After a 30 min starve and 30 min pulse period, one population of SV5 F mutants remained at 37°C for a 3 h chase period (A), while the other population was shifted to 30°C (B). Samples were immunoprecipitated and analyzed as above. Mutants that were processed at 30°C were expressed in Vero cells via the pCAGGS vector and metabolically labeled overnight at either 37°C (C) or 30°C (D), and surface biotinylation and analysis were performed as described above.