Table 1.

Surface expression of the SV5 CBF₁ mutants^a

Mutation	%Positiveb	M.F.I.c
Control (2°Ab only)	0.495	12.5
Mock	0.862	17.7
Wild-type SV5 F	100	100
G374A	7.53	17.4
N379A	0.977	19.1
I394A	4.93	21.1
Q396A	16.1	21.8
V402A	16.3	23.4
I405A	2.60	14.4
D406A	3.27	20.2

^aResults are representative of three separate experiments and normalized to percent wild-type SV5 F, set at 100%.

^bPercent cells positive for F protein expression, determined by flow cytometry as described in Experimental Procedures.

^cM.F.I., mean fluorescent intensity, determined by flow cytometry.