Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Aug 4;205(8):1903–1916. doi: 10.1084/jem.20080397

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Cosmi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — IL-17–producing cells can develop from UCB T cells in the presence of IL-1β plus IL-23. CD4⁺ T cells purified from UCB were stimulated for 7 d with anti-CD3 plus anti-CD28 mAb in the presence or absence of the indicated cytokines. (A) RORγt, T-bet, IL-23R, CD161, and IL-17 mRNA levels were measured by real-time quantitative RT-PCR. Bars represent mean values ± SE obtained from 13 donors. (B) Cells were also assessed by flow cytometry for their ability to produce IFN-γ alone (black bars), IL-17 alone (gray bars), or both (white bars) after stimulation with PMA plus ionomycin. Bars represent mean values ± SE obtained from 13 donors. (C) Representative flow cytometric analysis on UCB CD4⁺ T cells after stimulation with anti-CD3 plus anti-CD28 mAb, in the presence or absence of the indicated cytokines, performed after 1 wk (top) or 2 wk (bottom) of in vitro expansion. p-values refer to the comparison between cultures performed in the presence of the indicated cytokines versus those performed in the presence of medium alone. Percentages of gated cells are shown.