Skip to main content
. 2008 Aug 4;205(8):1917–1927. doi: 10.1084/jem.20071482

Figure 5.

Figure 5.

ESPs treated with GM6001 but not without GM6001 contribute to thrombus formation under flow conditions. (A) Schema of the experimental design. (B) Representative two-dimensional fluorescence images at 6 min. Glass slides coated with type I collagen were perfused at a wall shear rate of 1,500 s−1 for 6 min. Samples included reconstituted whole blood composed of ESPs (red, arrowheads) in blood obtained from 10–12 mice (per experiment), or a mixture of PE-labeled mouse platelets (red, arrowheads) in whole blood pooled from 10–12 mice. All murine platelets or ESPs were stained with mepacrine (green). ESPs capture fluorescent mepacrine as indicated by their dual expression of green and red by flow cytometer. Bars, 100 μm. (C) After 6 min, platelet thrombi formed on the collagen surface were quantified using NIH Images software from a recorded video. The results summarize four independent experiments (mean ± SEM).