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. 2008 Aug 4;205(8):1917–1927. doi: 10.1084/jem.20071482

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© 2008 Nishikii et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — The effect of metalloproteinase inhibition on posttransfusion recovery by ESPs in mice. (A) Schema of the experimental design. (B) 2 h after transfusion of 3 × 10⁶ ESPs, using a 129Ola strain mouse model in which platelet numbers are severely reduced, blood obtained from the retroorbital venous plexus was treated with 3.8% sodium citrate and subjected to flow cytometry to detect GFP⁺ platelets (ESPs) among all αIIb⁺ platelets. Additional serial evaluations were performed 24, 48, and 72 h after transfusion. Pretreatment with GM6001 increased the ratio of GFP⁺ ESPs per a recipient at all points examined. Results are the mean ± SEM from three independent experiments.