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. 2008 Aug 4;205(8):1889–1901. doi: 10.1084/jem.20072468

Figure 9.

Figure 9.

Twist1 knockdown increases inflammatory response in murine arthritis. (A) Experimental scheme. (B) Twist1 mRNA in 18-d-old Th1 expressing twist1-targeting shRNA or control shRNA restimulated with PMA/ionomycin. (C–E) Cell numbers of adoptively transferred GFP+ Th cells in spleen, mesenteric lymph nodes, and draining lymph nodes were analyzed on d 21 using FlowCount Beads. (F) Transfer of Th1 cells expressing a twist1-targeting shRNA leads to a significantly higher histological score in murine arthritis compared with control Th1 cells (d 21). Data are representative of two experiments.